Laser printing of DNA and protein microarrays The transfer of biomaterials, such as proteins, nucleic acids and oligonecleotides has a crucial role in the development of microarrays, biosensors, medical sensors and lab-on-chip devices. The printing of microarrays by liquid phase LIFT offers high spatial resolution, contact less approach and minimization of the biological material waste.
One of the most crucial steps for the photosynthetic amperometric sensors preparation is the immobilization of the photosynthetic material onto the electrodes surface. LIFT has been proven an advanced tool for direct immobilization of photosynthetic material onto low cost non-functionalized Au screen printed electrodes. The high kinetic energy of the transferred material enhances physical adsorption onto the electrode surface and results in high photocurrent activity by using extremely low quantities of photosynthetic material (thylakoid membranes).
The accuracy and the flexibility of LIFT technique enabled precise transfer of oligonecleotides probe sequences, CD 19 normal and CD 19 mutated, onto different membranes of a capacitive micro-array.
The micro-membrane arrays have been tested with a complementary oligonucleotide to the immobilized probe sequences at a concentration of 36 nM and measured significant real time response during the hybridization process.
